plasma mouse lipocalin  (R&D Systems)

Journal: eLife

Article Title: Reducing lipid bilayer stress by monounsaturated fatty acids protects renal proximal tubules in diabetes

doi: 10.7554/elife.74391

Figure Lengend Snippet: Figure 1. Saturated fatty acids high-fat diet (SFA-HFD) induces more tubular damage than monounsaturated fatty acids high-fat diet (MUFA-HFD) despite lower fat accumulation in mice. (A) Mice body weight throughout the experiment. The X-axis indicates the age of mice. (B) Schematic representation of tissue weight. (C) Plasmatic triacylglycerol (TAG) levels at week 16 after streptozotocin (STZ) injection. (D–F) Representative bright-field images of whole kidney sections (E) and cortex magnification (D) stained with Oil Red O (ORO). Quantification of the stained cortex area (F). Scale bars: 50 μm. (G) Plasmatic LCN2 levels at week 12 after STZ injection. (H, I) Representative bright-field images of kidney cortex stained with Picro-Sirius Red (H) and the quantification of the fibrotic cortex area (I). Scale bars: 50 μm. (J) Fibrotic area detected by Picro-Sirius Red normalized by fat deposition

Article Snippet: LCN2 plasma levels were determined by ELISA (R&D Systems, #AF1857- SP).

Techniques: Injection, Staining